富硒女贞子对热应激乳腺上皮细胞增殖、凋亡及抗氧化功能的影响

doi:10.11843/j.issn.0366-6964.2015.06.022

Abstract

Abstract:

The experiment was conducted to study the effects of se-enriched Ligustrum lucidium Fruit on proliferation，apoptosis and antioxidant activity of mammary epithelial cells cultured under heat circumstance in vitro.The heat stress model temperature was set as 42 °C and the temperature 37 °C was set as the control.In the experiment，heat-stressed mammary epithelial cells were cultured with different concentration of water extract，protein and oleanolic acid extract of Se-LLF.The growth and apoptosis rate of cultured mammary epithelial cells were detected by MTT and flow cytometry，respectively，and the content of SOD in the supernatant fluid of cultured mammary epithelial cells was detected by xanthine oxidase.The results showed that the proliferation of mammary epithelial cells cultured under high temperature was significantly promoted in each experimental concentration of water extract，protein and oleanolic acid extract of Se-LLF (P<0.05).The apoptosis rate decreased significantly (P<0.05)，the activity of SOD (P<0.05) was increased significantly，and the results were better than those of the ordinary Ligustrum lucidum group.In conclusion，water extract，protein and oleanolic acid extract of Se-LLF can not only improve the proliferation and apoptosis rate of heat-stressed mammary epithelial cells，but also improve their antioxidant capacity.

CLC Number:

S853.74

ZOU Hui，LI Dan-dan，CHEN Jing，LAO Xue-fen，CAO Zheng，SHANG Qiu-chen，XU Xiao-qin. Influence of Se-enriched Ligustrum lucidium Fruit on Proliferation，Apoptosis and Antioxidant Activity of Mammary Epithelial Cells under Heat Stress[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(6): 1047-1054.

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Influence of Se-enriched Ligustrum lucidium Fruit on Proliferation，Apoptosis and Antioxidant Activity of Mammary Epithelial Cells under Heat Stress

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